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KMID : 0380619900220050520
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Korean Journal of Food Science and Technology
1990 Volume.22 No. 5 p.520 ~ p.525
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Selective Enrichment of EPA and DHA by Adduct Formation of Fatty Acids of Fish Oil and Urea
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신현경/Shin, Hyun Kyung
안병학/한대석/Ahn, Byung Hak/Han, Daeseok
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Abstract
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A modified method of urea adduct formation, in which water and organic solvent were used as the wetting agent and the reaction medium, respectively, is suggested to obviate methanolysis and to ease recovery in the separation of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) from fish oil. With the new method the fraction in which the total content of EPA, DHA and their precursors is more than 80% could be obtained. Although the total content of precursors in the concentrate was hardly affected by the kind of the wetting agent or the organic solvent, the content of E PA and DHA varied significantly depending on the organic solvent. This finding made it possible to selectively enrich the desired components. After DHA-enriched fraction (DHA is 50%) was obtained by wing pentane, EPA-enriched fraction (EPA is 53%) could be obtained from the residue of DHA-enriched fraction by using heptane.
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